报告题目:Next generation cell culture system for HT dr
新闻类型:学术活动  阅读次数:  发布时间:2017-07-06

报告题目:Next generation cell culture system for HT drug screening, tissue engineering and regenerative medicine

  人:Lin Zhe(苏黎世联邦理工学院Department of Health Sciences and Technology, ETH Zurich, CH- 8093, Zurich, Switzerland

报告时间:2017708日上午9:00-12:00

报告地点:福州大学阳光科技楼北905会议室

报告摘要:Despite the crucial role of extracellular matrix (ECM) in directing cell fate in healthy and diseased tissues—particularly in development, wound healing, tissue regeneration and cancer—the mechanisms that direct the assembly and regulate hierarchical architectures of ECM are poorly understood. Collagen I matrix assembly in vivo requires active fibronectin (Fn) fibrillogenesis by cells. Here we exploit Fn-FRET probes as mechanical strain sensors and demonstrate that collagen I fibres preferentially co-localize with more-relaxed Fn fibrils in the ECM of fibroblasts in cell culture. Fibre stretch-assay studies reveal that collagen I’s Fn-binding domain is responsible for the mechano-regulated interaction. Furthermore, we show that Fn-collagen interactions are reciprocal: relaxed Fn fibrils act as multivalent templates for collagen assembly, but once assembled, collagen fibres shield Fn fibres from being stretched by cellular traction forces. Thus, in addition to the well-recognized, force-regulated, cell-matrix interactions, forces also tune the interactions between different structural ECM components.

 报告人简介:Dr. Lin Zhe is a member of the Department of Health Science and Technology within the Laboratory of Applied Mechanobiology at the ETH Zürich, Switzerland. The Applied Mechanobiology Laboratory exploits nanotechnology tools to decipher how bacteria, mammalian cells, and micro-tissues take advantage of mechanical forces to recognize and respond to material properties in their native environments. Their overarching goal is to discover mechanisms how nature exploits mechanical forces as an additional dimension of functional regulation and how these insights can be exploited for biomedical applications and in regenerative medicine. This includes asking how the mechanobiology of extracellular matrix directs stem cell differentiation and (micro)tissue growth and functions. Also bacteria sense mechanical forces which regulate their adhesion to surfaces and tissue fibers, and immune cells use mechanical forces to fight bacterial infections. The discoveries of the Lab in single molecule and cell mechanics and how protein stretching switches their function, have a wide range of technical and medical implications. In collaboration with clinicians, several technologies are currently carried towards preclinical studies. Their future goal is to exploit their interdisciplinary strength in basic research, while translating key discoveries towards the clinic.

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